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Registros recuperados: 12
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Balneola vulgaris gen. nov., sp nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea ArchiMer
Urios, Laurent; Agogue, Hélène; Lesongeur, Francoise; Stackebrandt, Erko; Lebaron, Philippe.
A novel aerobic, Gram-negative bacterium, named 13IX/A01/164(T), was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were motile, straight rods, 2-5 mu m long and 0-2 mu m wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164T was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum Bacteroidetes within the family Crenotrichaceae. On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name Balneola vulgaris gen. nov., sp. nov. is proposed. The type strain of Balneola vulgaris is 13IX/A01/164T (=DSM 17893T=CIP...
Tipo: Text Palavras-chave: Mediterranean sea; Coastal waters; Microbiology; Physiology; Biochemistry; Gene sequence; Balneola vulgaris.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2126.pdf
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Biodiversité et dynamique des communautés biologiques en milieu marin ArchiMer
Fromentin, Jean-marc; Aliaume, Catherine; Lebaron, Philippe.
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00486/59816/62959.pdf
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Distinct bacterial groups contribute to carbon cycling during a naturally iron fertilized phytoplankton bloom in the Southern Ocean ArchiMer
Obernosterer, Ingrid; Catala, Philippe; Lebaron, Philippe; West, Nyree J..
We investigated the contribution of distinct bacterial groups to bulk abundance and leucine incorporation during a spring phytoplankton bloom induced by natural iron fertilization in the Southern Ocean (Kerguelen Ocean and Plateau Compared Study, January-February 2005). Oligonucleotide probes were designed to target five operational taxonomic units (OTUs) at a narrow phylogenetic level (>= 99% identity of the 16S ribosomal ribonucleic acid [rRNA] gene). During the peak of the phytoplankton bloom, the Roseobacter groups NAC11-7 and RCA, the OTUs SAR92 belonging to Gammaproteobacteria, and the Bacteroidetes OTU Agg58 dominated bulk abundance and leucine incorporation. These four OTUs disappeared with the decline of the bloom, when the cosmopolitan groups...
Tipo: Text
Ano: 2011 URL: https://archimer.ifremer.fr/doc/00214/32503/30995.pdf
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Diversity of quorum sensing autoinducer synthases in the Global Ocean Sampling metagenomic database ArchiMer
Doberva, Margot; Sanchez-ferandin, Sophie; Toulza, Eve; Lebaron, Philippe; Lami, Raphael.
Quorum sensing (QS) is a cell-to-cell signalling pathway that allows bacteria to synchronize their genetic expression. It is mediated by autoinducers (AI), including (1) acyl-homoserine lactones (AHLs or AI-1), produced by Proteobacteria using AinS, LuxI and HdtS synthase families and (2) furanosyl-diester-borate (FDB or AI-2), produced by a large range of phylogenetically diverse bacteria and synthetized by the LuxS family. Few data have been collected about the presence and importance of QS in marine waters using culture independent methods. In this study, we examined the presence and the diversity of AI-1 and AI-2 synthases in the Global Ocean Sampling (GOS), a large metagenomic database, covering 68 stations across 3 oceans. We built 4 reference...
Tipo: Text Palavras-chave: Quorum sensing; Acyl-homoserine lactone synthases; Global Ocean Sampling; Environmental metagenomes.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00615/72718/71710.pdf
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Enhanced heterotrophic activity in the surface microlayer of the Mediterranean Sea ArchiMer
Obernosterer, Ingrid; Catala, Philippe; Reinthaler, Thomas; Herndl, Gerhard J.; Lebaron, Philippe.
The abundance and activity of heterotrophic and autotrophic organisms were determined in the surface microlayer (SML) at marine and estuarine sites in the NW Mediterranean Sea from May to July 2003. At all study sites, community respiration in the SML exceeded that in the subsurface water (SSW) by factors between 1.7 and 28. The abundance of heterotrophic bacteria and the major groups of the autotrophic community displayed consistent, but low enrichment factors (EF) for the SML (mean 1.3). In contrast, EF for the abundance of heterotrophic nanoflagellates and the concentration of particulate organic carbon were, on average, 4 and 7, respectively. Community respiration rates in the SML were related to the concentration of total organic carbon; however, no...
Tipo: Text Palavras-chave: Surface microlayer; Community respiration; Net community production; Neuston.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/00243/35435/33960.pdf
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Microbial community structure in the sea surface microlayer at two contrasting coastal sites in the northwestern Mediterranean Sea ArchiMer
Joux, Fabien; Agogue, Helene; Obernosterer, Ingrid; Dupuy, Christine; Reinthaler, Thomas; Herndl, Gerhard J.; Lebaron, Philippe.
In an attempt to compare the microbial community structure between the sea surface microlayer (SML) and subsurface waters (SSW), we determined the enrichment factors (EF: the ratio of abundance or activity in the SML to abundance or activity in SSW) of 13 biological parameters. Samples were taken at 2 contrasting coastal sites in the Mediterranean Sea, corresponding to a high (Barcelona, Spain) and low (Banyuls-sur-Mer, France) urbanized area. Principal component analysis showed that temporal variability was much higher at Barcelona than at Banyuls, and that the characteristics of the SML and SSW samples were more closely related at Barcelona. At both sites, the SML was weakly enriched in heterotrophic bacteria (on average 1.1-fold), Synechococcus spp. (on...
Tipo: Text Palavras-chave: Sea surface microlayer; Enrichment factor; Microbial structure; Virus-like particles; Bacteria; Microalgae; Nanoflagellates.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/00243/35434/33961.pdf
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Moose: An integrated multi-sites observatory system in the NW Mediterranean sea ArchiMer
Mantoura, F; Coppola, Laurent; Cousin, M.; Antoine, David; Beguery, Laurent; Blain, Stephane; Bouin, Marie-noelle; Cadiou, Jean-francois; Durrieu De Madron, Xavier; Forget, Philippe; Fraunie, Philippe; Gojak, Carl; Gorsky, Gaby; Goyet, Catherine; Lebaron, Philippe; Lellouche, Jean-michel; Ludwig, Hubert; Molcard, Anne; Mortier, Laurent; Petit De La Villeon, Loic; Petrenko, Anne; Piazzola, J.; Queguiner, B.; Raimbault, Patrick; Rigaud, Vincent; Sempere, Richard; Tamburini, Jean-simon; Testor, Pierre; Vuillemin, Renaud; Zakardjian, B..
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00122/23341/21166.pdf
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Répartition et dynamique de différentes populations bactériennes autochtones et allochtones dans les eaux de l'étang de Thau ArchiMer
Lebaron, Philippe.
The purpose of this study is to investigate the behavior of two bacterial communities in the Thau brackish lagoon (Mediterranean coast of France) : the bacterial community from continental origin (allochtonous community) and the bacterial community endemic to the lagoon (autochtonous community). In a first step, the ability of two standard agar media (bioMerieux, nutrient agar at low salinity, 8; Difco, marine agar at high salinity, 34) to distinguish the two communities is tested. Then, abundances sampling of these two bacterial variables for several scales of observation permit to describe their spatial and temporal evolutions. The different encountered behaviors allow us (i) to improve the definition of an optimal sampling design for such bacterial...
Tipo: Text
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00073/18379/15952.pdf
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Responses of enteric bacteria to environmental stresses in seawater ArchiMer
Troussellier, Marc; Bonnefont, Jean-luc; Courties, Claude; Derrien, Annick; Dupray, Elizabeth; Gauthier, Michel; Gourmelon, Michele; Joux, Fabien; Lebaron, Philippe; Martin, Yvan; Pommepuy, Monique.
The effects of different environmental factors (nutrient deprivation, hyperosmotic shock, exposure to light) on enteric bacteria which have been transferred into the marine environment, have been studied experimentally (microcosms) by considering demographic, physiological and genetic responses in Escherichia coli or Salmonella typhimurium populations. Short-term experiments (less than or equal to 48 h) showed that nutrient deprivation induced limited changes in measured bacteriological variables, but when combined with hyperosmotic shock, it results in an energy charge decrease and inactivation of membrane transport. Light exposure mainly affects the colony-forming capacity of bacterial populations. Combining different stress factors confirmed the rapid...
Tipo: Text Palavras-chave: Genetics; Cellular states; Physiology; Seawater; Enteric bacteria; Génétique; Etats cellulaires; Physiologie; Eau de mer; Entérobactéries.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-845.pdf
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Seasonal dynamics of active SAR11 ecotypes in the oligotrophic Northwest Mediterranean Sea ArchiMer
Salter, Ian; Galand, Pierre E.; Fagervold, Sonja K.; Lebaron, Philippe; Obernosterer, Ingrid; Oliver, Matthew J.; Suzuki, Marcelino T.; Tricoire, Cyrielle.
A seven-year oceanographic time series in NW Mediterranean surface waters was combined with pyrosequencing of ribosomal RNA (16S rRNA) and ribosomal RNA gene copies (16S rDNA) to examine the environmental controls on SAR11 ecotype dynamics and potential activity. SAR11 diversity exhibited pronounced seasonal cycles remarkably similar to total bacterial diversity. The timing of diversity maxima was similar across narrow and broad phylogenetic clades and strongly associated with deep winter mixing. Diversity minima were associated with periods of stratification that were low in nutrients and phytoplankton biomass and characterised by intense phosphate limitation (turnover timeo5 h). We propose a conceptual framework in which physical mixing of the water...
Tipo: Text
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00374/48560/48969.pdf
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Spatial comparison of total vs. active bacterial populations by coupling genetic fingerprinting and clone library analyses in the NW Mediterranean Sea ArchiMer
Rodriguez-blanco, Arturo; Ghiglione, Jean-francois; Catala, Philippe; Casamayor, Emilio O.; Lebaron, Philippe.
Spatial distributions of both total (i.e. 16S rDNA-based fingerprints) and active (i.e. 16S rRNA-based fingerprints) bacterial populations, together with total bacterial activity measured by (3)H-leucine incorporation, were studied along a 98 km transect in the NW Mediterranean Sea. Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) fingerprinting was coupled to a clone library, allowing CE-SSCP peaks identification and the monitoring of the spatial variation of bacterial phylotypes. Up to 80% of the community peaks matched those obtained from clone library sequences, accounting for 86.7% of the total fingerprinting area. A good agreement was found between the relative abundance of Prochlorococcus in the CE-SSCP fingerprints and...
Tipo: Text Palavras-chave: CE-SSCP fingerprinting; 16S rRNA; Spatial distribution; Bacterial diversity and activity Prochlorococcus; SAR11; Gammaproteobacteria.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00243/35433/33965.pdf
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Thalassobaculum salexigens sp nov., a new member of the family Rhodospirillaceae from the NW Mediterranean Sea, and emended description of the genus Thalassobaculum ArchiMer
Urios, Laurent; Michotey, Valerie; Intertaglia, Laurent; Lesongeur, Francoise; Lebaron, Philippe.
A novel Gram-negative bacteria, named CZ41_10a(T), was isolated from coastal surface waters of the north-western Mediterranean Sea. Cells were motile, pleomorphic rods, 1.6 mu m long and 0.7 mu m wide and formed cream colonies on marine agar medium. The G+C content of the genomic DNA was 65 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the new isolate in the genus Thalassobaculum, a member of the family Rhodospirillaceae, class Alphaproteobacteria. Unlike Thalassobaculum litoreum CL-GR58(T), its closest relative, strain CZ41_10a(T) was unable to grow anaerobically and did not exhibit nitrate reductase activity. On the basis of DNA-DNA hybridization, fatty acid content and physiological and biochemical characteristics, this isolate...
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00001/11201/7846.pdf
Registros recuperados: 12
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